Micropipette activity

Printable version: pipette activity
This activity is designed to be done with RAININ LTS micropipettes.

Teachers: Set up two stations in your classroom. At each station place lab markers, and several beakers of colored water.

At station #1 place 1.5ml microcentrifuge tubes and RAININ micropipettes L-20, L-200, and L-1000 on the table.

At station #2 place pre- cut pieces of waxed paper or parafilm and RAININ micropipettes L-2 and L-20 on the table.

Station #1

  1. Fill in the blanks on the following chart:
Reaction Tube A Solution 1 Solution 2 Solution 3 Solution 4 Solution 5
Volume: 15 µL 105 µL 12 µL 38 µL 350 µL
Micropipette:(L-20, L-200, L-1000) L-_ L-_ L-_ L-_ L-_
Micropipette setting
  1. Select a 1.5ml tube and label it TUBE A with your initials.
  2. Pipet each of the volumes indicated above into Tube A, using the correct micropipette.
  3. To check your accuracy, add the volumes of solutions 1-5 = _ µL.
  4. Select the appropriate pipette to measure this volume: L-_.
  5. Set the pipette to the total volume and withdraw all of your liquid from Tube A.

The contents should just fill your tip with no air space at the bottom of the tip and no liquid left in the tube. Discard tip and tube into waste container.

Station #2

  1. Fill in the blanks on the following chart:
Waxed Paper Solution 1 Solution 2 Solution 3 Solution 4 Solution 5
Volume: 0.5 µL 10.0 µL 3.0 µL 1.0 µL 8.0 µL
Micropipette:(L-2, L-20) L-_ L-_ L-_ L-_ L-_
Micropipette setting
  1. Select a piece of wax paper and label it with your initials.
  2. Carefully pipet each volume onto the waxed paper, in the order indicated above.
  3. Compare the solution drops with those of other students in the class.
  4. Have your teacher view and check your results.