Objectives:
- Identify a space appropriate for a molecular biology laboratory
- Evaluate any potential challenges in setting up your lab
A working genetics lab can be set up in almost any standard classroom science area.
There are a number of considerations when picking a room
- Ventilation
- Some chemicals can be harmful to inhale and some have flammable vapors. Choose a space with some airflow.
- Molecular biology experiments are sensitive to contamination from dust, pollen, etc. Close windows and avoid fans and blowing air.
- Temperature
- Room temperature is considered 20-25 °C or 68-77 °F
- Many of the chemicals in the kits are best stored at room temperature and you will often be instructed to incubate reactions at room temperature.
- If the temperature of the room is too cold, precipitates or crystals may form.
- Some reactions may not performed efficiently.
- If necessary, the circulating water bath can be set to 20-25 °C and room temperature reactions can be carried out.
- Lighting
- A good light source is important as you’ll be working with small items and small quantities.
- However, chemical are light-sensitive, especially to UV. Don’t store chemicals in sunlight. If necessary, cover containers with aluminum foil.
- Other considerations
- Linoleum floor: easy to clean, doesn’t absorb spilled chemicals
- Security: some of the equipment and supplies can be hazardous if handled improperly. Make sure everyone who uses the room is aware of basic laboratory safety rules. Lock cabinets!
There are a number of characteristics to look for when picking out a work area, or Lab Bench.
- Non-reactive surface: your lab bench shouldn’t absorb liquids and be easy to clean. A bench could also be covered with a heavy garbage bag for protection.
- Sturdy: some equipment, such as the centrifuge, require a steady, stable base.
- Electrified: the bench should be located near electric outlets as extension cords running across the floor are a tripping hazard.
- Ideally, a bench should be spacious. Some precision equipment, such as the scale, are sensitive to being moved and should have a permanent home on the bench-top.
- Ideally, a bench should be near a sink. For the safety of yourself and others, hands should be washed immediately after removing gloves and before touching anything else.
Other furniture
- Shelves: for storage and organization of frequently used items and supplies
- Cabinet: keeps things secure and dust-free when they’re not being used. Store chemicals in the cabinet to keep them out of the light.
Other equipment, regular househole versions of
- Microwave
- Refrigerator
- Freezer
NO FOOD! Put up signs to keep people from using them for food preparation and storage.