DNA Extraction Video

Video text:

  • The purpose of DNA Extraction is to get pure DNA out of your sample.
  • In this video, you will learn how to use an extraction kit. This kit is for soil.
  • The kit contains the chemicals and supplies you need to extract DNA.
  • The first time you use the kit, add ethanol to the concentrated buffers.
  • Be sure to mark the tops of the buffer bottles.
  • Before starting, if a heat block is needed, turn it on and set it to the correct temperature.
  • Label the tops of the spin columns and microcentrifuge tubes.
  • The first step is LYSIS. This breaks open the cell to free the DNA.
  • If extracting DNA from tissue, add Buffer ATL and proteinase K. Follow specific directions if extracting from plants or soil.
  • Vortex the sample to mix.
  • Place in heating block until tissue is completely dissolved. Vortex occasionally.
  • When sample is completely lysed, vortex for 15 seconds.
  • Add first Wash Buffer. The wash buffers precipitate DNA and rinse away proteins.
  • Pipet into a labeled spin column labeled. Spin columns have a filter that binds DNA.
  • Place samples in centrifuge and secure cover before closing lid.
  • Centrifuge samples for recommended speed and time.
  • Discard the flow-through and old collection tube. Place spin column in a new collection tube.
  • Add second wash buffer.
  • Centrifuge samples for recommended speed and time.
  • Place column in a clean, LABELED 1.5ml microcentrifuge tube. Pipet Elution Buffer or water directly onto the DNEasy membrane.
  • Incubate for 1 minute to extract DNA.
  • Centrifuge samples for recommended speed and time.